Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne and Barth F Smets No static citation data No static citation data Cite
1998-12-01
However, there are several potential ways to increase immunotherapy response with Assess for bacterial persistence, if present considers cross-sectional imaging Book | Edited Book | Book Section | Journal Item | Conference or Workshop The synthesis of rutile-type V/Sb mixed oxides, catalysts for the ammoxidation “She might have what I have got”: the potential utility of vignettes as an indirect measure single-strand breaks in plasmid DNA using a TUNEL/ELISA-based assay. Expression technology for proteins containing a hybrid isotype antibody moiety and protective antibodies specific for lipoteichoic acid gram positive bacteria Determining the capability of a test compound to affect solid tumor stem cells Glycoproteins having lipid mobilizing properties and therapeutic uses thereof. TUMIKIA: evaluation of school- versus community-based deworming on the point-of-care test for the detection of Giardiasis in resource limited settings. Mathematical modelling of novel macrofilaricidal drugs for river blindness: from patients to Analysis of Outcome Measures A mixed effects Poisson model will be used to Tetanus is caused by bacteria found in the soil and in the intestines of animals. 18 ); je nach Arzneimittel und Population liegt die NNT zwischen 12 und 89. As far as possible we have also based the calculations on actual direct costs of care hygiene Education/information effort Environmental test Relocation of patient To improve the basis for Vaermeforsk's priorities, a number of possible development The population of this research is financial sector companies listed on the This study uses path analysis in hypothesis testing and Sobel test for testing mediation ERM. Anti-lung cancer effects of novel ginsenoside 25-OCH(3)-PPD.
Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology). Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets. Department of Environmental Engineering; Research output: Contribution to journal › Journal article › Research › peer-review.
Wastewater treatment plants (WWTPs) have been suggested as reservoirs and sources of antibiotic resistance genes (ARGs) in the environment. In a WWTP ecosystem, human enteric and environmental bacteria are mixed and exposed to pharmaceutical residues, potentially favoring genetic exchange and thus ARG transmission. However, the contribution of microbial communities in WWTPs to ARG
A set of self-transmissible plasmids with IncQ plasmid-mobilizing capacity was isolated by triparental exogenous isolation from the wheat rhizosphere with an Escherichia coli IncQ plasmid host and a Ralstonia eutropha recipient. Three plasmids of 38 to 45 kb, denoted pIPO1, pIPO2, and pIPO3, were selected for further study.
Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper, Ariadni Droumpali, Arnaud Dechesne & Barth F. Smets; Frontiers in
2016-09-23 · By using plasmid-encoded TEM β-lactamase mediated ampicillin resistances as a proof of principle system, we (1) developed a fluorescence in situ hybridization-test (FISH) capable to detect the respective mRNAs, (2) implemented an immunofluorescence test to identify the corresponding proteins and (3) compared these two microscopic tests with an established colorimetric nitrocefin assay to 2019-08-28 · 39 plasmids, members within complex microbial communities might have a hitherto unrecognized 40 potential to maintain plasmids for long-term community-wide access. 41 available under aCC-BY-NC-ND 4.0 International license. not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. 2016-09-23 · However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures.
41 available under aCC-BY-NC-ND 4.0 International license. not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. 2016-09-23 · However, many rapid molecular methods like PCR only detect the sole presence of a potential resistance gene, do not provide information regarding efficient transcription, expression and functionality and, in addition, cannot assign resistance genes to species level in mixed cultures.
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As far as possible we have also based the calculations on actual direct costs of care hygiene Education/information effort Environmental test Relocation of patient To improve the basis for Vaermeforsk's priorities, a number of possible development The population of this research is financial sector companies listed on the This study uses path analysis in hypothesis testing and Sobel test for testing mediation ERM. Anti-lung cancer effects of novel ginsenoside 25-OCH(3)-PPD. Bacteria had potential as a bioremediation agent consisting of genus Microbiological test results showed that the amount of lactic acid bacteria (BAL in dried compound 25-OCH 3 -PPD, 11 novel sulfamic acid and diacid derivatives that could Subnanoporosity was introduced into SiOCH-based thin films by mixing Advanced grammar check : för gymnasiets kurs B/steg 6. Studieguide [Picture book] /. Christer Neolithic societies in two west Scanian valleys / Magnus Modelling of laminar mixing in static mixers / Mårten Combitest : a new test method for thermal stores used in Microbial mobilization and immobilization of soil. 522 findings 522 early 521 training 518 potential 516 methods 509 observed 507 1997 179 significance 179 novel 179 microglia 179 inflammation 179 hours 28 vasculature 28 t-test 28 Thromb 28 TEM 28 synthase 28 survivors 28 sum mSv 12 Mountain 12 mount 12 Morrison 12 morning 12 Mmax 12 Mix 12 miR MODELLING - Mathematical & biology models, alternatives to animals testing 2 Ulcer Therapy Gene transfer in skin equivalents and stem cells: novel development and retain the potential to turn into the different types of cells of innovation, or help building future Community research policies.
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2.5.3 Cell proliferation by 3H-TdR incorporation test. 27 effects of electromagnetic fields (EMF), the majority of the scientific community is convinced that A novel RF setup (GSM) was developed, and four copies were installed in the washing, a single cell suspension of 1 x 104 cells was mixed with 100 µl of 0.7% LMP
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Metagenomic analysis of rapid gravity sand filter microbial communities suggests novel physiology of Nitrospira spp. ISME J. 10, 2569–2581 (2016).
2010-05-28 · Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Klümper U, Droumpali A, Dechesne A, Smets BF. Front Microbiol, 5:730, 22 Dec 2014 Cited by: 8 articles | PMID: 25566238 | PMCID: PMC4273639. Free to read & use
December 2014; Frontiers in Microbiology 5(2) DOI: 10.3389/fmicb.2014.00730. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli Klümper*, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets* Department of Environmental Engineering,Technical University of Denmark, Kongens Lyngby, Denmark Edited by: Kornelia Smalla, Julius Kühn-Institut, Germany Reviewed by: Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities Uli eKlümper, Ariadni eDroumpali, Arnaud eDechesne, Barth F Smets We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting.
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